The Molecular Human – A Roadmap of Molecular Interactions Linking Multiomics Networks with Disease Endpoints.
What/Who is “The Molecular Human”?
The Molecular Human is a network of over 34,000 associations between over 8,700 multiomics traits and disease endpoints. The underlying associations are derived from 18 technically diverse deep molecular phenotyping (omics-)platforms analyzing urine, blood, and saliva samples from up to 374 participants of the multi-ethnic diabetes case-control study QMDiab [click here to read the QMDiab cohort paper].
The links between the multiomics traits include (1) partial correlations between traits from the same platform (GGMs), (2) mutual best hits for pairwise all-against-all correlations between platforms, (3) genome-wide, epigenome-wide and transcriptome-wide associations with multiomics traits and disease endpoints, and (4) phenome-wide associations with QMDiab clinical traits.
Below are a few screenshots of what The Molecular Human is and provides:
What can be learned from The Molecular Human?
We are presently in the process of writing the paper that describes The Molecular Human. Once it is ready we shall post it on MedRxiv and link it here.
In the meantime we are using this platform to actively post individual analyses, insights and use-cases using The Molecular Human and in particular its comics web-interface. Here are the posts that are available so far:
Which are the omics platforms used to characterize The Molecular Human?
|DNA||GENOMICS||Genotype||Infinium Human Omni 2.5-8 V1.2 Beadchip array||DNA extracted from buffy coat fraction from whole blood|
|MET||METHYLOMICS||DNA methylation||Illumina Infinium HumanMethylation450 (450K) BeadChip array||DNA, same as for genomics|
|RNA||TRANSCRIPTOMICS||Gene expression||RNA sequencing based Illumina ~20M reads.||RNA extracted from PaxTubes|
|miRNA||TRANSCRIPTOMICS||microRNA expression||microRNA profiling based multiplex qPCR, Exiqon.||RNA extracted from EDTA plasma|
|SOMA||PROTEOMICS||Protein abundance||Slow Off-rate Modified Aptamer based SomaScan, Somalogic 1,1k.||EDTA plasma|
|OLINK||PROTEOMICS||Protein abundance||High-multiplex immunoassays based Olink Metabolism & Cardiometabolism assays.||Heparin plasma|
|PGP||GLYCOMICS||Total plasma N-glycosylation||Hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) based Genos pipeline.||EDTA plasma|
|IgG||GLYCOMICS||IgG glycosylation||Liquid chromatography mass spectrometry (LC/MS) based on Ginos pipeline.||EDTA plasma|
|IgA||GLYCOMICS||IgA & IgG glycosylation||LC/MS based on Wuhrer lab pipeline.||EDTA plasma|
|BRAIN||LIPOPROTEOMICS||Lipoproteins||Proton nuclear magnetic resonance (1H NMR) based Nightingale technology.||EDTA plasma|
|LD||LIPIDOMICS||Lipid concentration||LC/MS based on Lipidyzer technology at Metabolon.||EDTA plasma|
|BM||LIPIDOMICS||Lipids and other metabolite concentration||Flow injection analysis mass spectrometry (FIA/MS) based Biocrates technology.||EDTA plasma|
|HDF||METABOLOMICS||Metabolite level||Hydrophilic Interaction Liquid Chromatography mass spectrometry (HILIC/MS) & Ultra-performance liquid chromatography mass spectrometry (UPLC/MS) based HD4 Metabolon.||EDTA plasma|
|PM||METABOLOMICS||Metabolite level||Gas chromatography mass spectrometry (GC/MS) & Ultra-performance liquid chromatography mass spectrometry (UPLC/MS) based HD2 Metabolon.||EDTA plasma|
|SM||METABOLOMICS||Metabolite level||Gas chromatography mass spectrometry (GC/MS) & Ultra-performance liquid chromatography mass spectrometry (UPLC/MS) based HD2 Metabolon.||Saliva|
|UM||METABOLOMICS||Metabolite level||Gas chromatography mass spectrometry (GC/MS) & Ultra-performance liquid chromatography mass spectrometry (UPLC/MS) based HD2 Metabolon.||Urine|
|CM||METABOLOMICS||Metabolite level||1H NMR deploying Chenomx for annotation, based on Univ. Greifswald pipeline.||Urine|
|CLIN||CLINICAL||Clinical biochemistry and blood counts||Cobas 6000; Roche Diagnostics||Blood/Urine|